Functional synthetic mRNA may be obtained by in vitro transcription of a cDNA template, typically plasmid DNA (pDNA), using a bacteriophage RNA polymerase. The preparation of pDNA is the first step in the production of mRNA. Manufacture of mRNA might thus appear to require more effort than manufacture of pDNA. However, unpolished pDNA contains traces of bacterial genomic DNA and three forms of pDNA (supercoiled, relaxed circle or linear) in variable proportions. Linearized pDNA is transcribed in many cases using bacteriophage RNA polymerase, because all DNA is removed during further processing steps.
We offer the enzyme tools required for mRNA research and production, involving synthesis, purification, capping.
Deoxyribonuclease I (DNase I)
T4 RNA ligase I (ssRNA ligase)
T7 RNA Polymerase
RNase inhibitor (Recombinant)
Alkaline Phosphatase, TAB5
Pyrophosphatase Inorganic (yeast)
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